ReconFlex

Localization microscopy in general is time and disk space consuming by recording an image stack comprising tens of thousands of images for post-processing of the data to determine the position of each molecule (reconstruction).

 

ReconFlex™ supports a much faster data analysis for localization microscopy.

Advantages

Enormous Reduction in Data Transfer, Time and Disk Space

Select Fiducial Markers prior to the Measurement to correct Possible Drifts in Real-Time

The User retains Full Control over the Algorithms used in the Reconstruction Mode

"On Board" Localization of Local Intensity Peaks (so called "Blobs"), e.g. Coordinate Measurements of Thousands of Local Blobs per Frame without Latency Time

 

   Ideal for Phosphor-Screen or Scintillation Readout

 

   Perfect Solution for Blinking Super-Resolution Microscopy

 

Very Fast and very Accurate Time Stamping of Blob Coordinates

 

"On Board" Accumulation of taken Pictures

 

Image Quality very close to well known sCMOS Camera Types

 

High Frame Rates with very High Dynamics

Provided with ReconFlex™, nanoFLeye™ opens up a new door to real-time, easy and user-friendly super-resolving localization microscopy.

It offers ultra-high flexibilty in terms of its different modes:

Normal Camera Mode: For  Live Imaging, adjusting the Microscope and recording Imaging Stacks

Reconstruction Support Mode: A Fast Detection Mode of Localization Areas by the nanoFLeye™ System

CAMERA MODELS

General specifications of the ReconFlex™ 1900-Series:

 

  sensor technology: CMOS (global shutter)

  optical format: 1/1.2”

  pixel size: 5.86µm

  resolution: 1920 x 1200 (2.3Mpixel)

  quantum efficiency: >75% (@ 525nm)

  read noise: <6e-

  saturation capacity: 32500e-

  dynamic range: 75dB

  frame rate (max. @ full frame): 164fps (@ADC 10bits)

  digital dynamic range sampling up to 32bit

  signal-to-noise ratio: 45dB

  exposure time (min.): 20µs

  trigger in/out: LVTTL (<6µs precision)

 

  data interface: USB3.0

USB3.0 driver for Windows(R)10, 8, 7 and Linux 64bit operation systems

compatible to the Surface Concept universal software interface

Time-to-Digital

Converter

Description

Applications

ReconFlex™ 1900

 

 

 

 

 

 

 

 

 

ReconFlex™ 1900T

 

 

 

 

 

 

 

 

 

 

 

ReconFlex™ 1900S

 

 

 

 

 

 

 

 

 

ReconFlex™ 1900ST

 

 

Camera system with integrated blob localization (normal resolution) and high precision trigger system (<6µs)

 

 

 

 

 

Camera system with integrated blob localization (normal resolution), high precision trigger system (<6µs) and integrated time to digital converter (TDC, 82ps)

 

 

 

 

 

Camera system with integrated latency free super-resolution microscopy mode based on the ReconFlex™ 1900

 

 

 

 

 

Camera system with integrated latency free super-resolution microscopy mode with TDC based on the ReconFlex™ 1900T

 

Production level estimated December 2019

Coming soon (under developement): ReconFlex™ 800-Series

 

   High frame rate cameras (up to 1595Hz @ full frame (800x600pixel))

   Integrated normal and super-resolution blob localization and time correlation

 

ReconFlex™ 800

ReconFlex™ 800T

ReconFlex™ 800S

ReconFlex™ 800ST

 

These 4 models are corresponding in their special functionality to the 4 models of the ReconFlex™ 1900-Series.

 

Production level estimated from 1st quarter 2020.

Super-Resolution

Microscopy Mode

  • fast and time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • time correlated microscopy with resolutions of 6μs or above
  • very fast „on Board“ localization microscopy with non-interpolated resolution (at sensor pixel resolution)
  • machine vision

 

  • ultra fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • ultra fast time correlated microscopy with resolutions of 82ps or above (signals to the TDC can drive the image sensor shutter)
  • ultra fast time correlated „on Board“ localization microscopy with non-interpolated resolution (at sensor pixel resolution)
  • machine vision

 

  • real time SPDM, STORM, PALM super-resolution localization microscopy (up to 16 times below the sensor pixel resolution)
  • fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • time correlated microscopy with resolutions of 6μs or above
  • machine vision

 

  • real time SPDM, STORM, PALM super-resolution localization microscopy (up to 16 times below the sensor pixel resolution)
  • ultra fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • ultra fast time correlated microscopy with resolutions of 82ps or above (signals to the TDC can drive the image sensor shutter)
  • machine vision

APPLICATIONS

Localization Microscopy

Localization microscopy in general is time and disk space consuming by recording an image stack comprising tens of thousands of images for post-processing of the data to determine the position of each molecule (reconstruction).

 

The „Super-resolution Mode“ enables the true localization of single molecules. The locations are determined by the integrated algorithm with a precision down to 20nm.

Blob Finder Mode

The Blob Finder Mode enables the true counting of single light blobs. This evaluates the positions independently on the fluctuations of the MCP amplification. Thus, the Blob Finder Camera is capable of linear rate response down to the lowest count rate range.

 

Frame triggers are programmable by software or follow external TTL signals. These TTL signals can be measured as a third coordinate with a precision of 82ps with respect to a laboratory reference clock.

Timeresolved Imaging

 

SOFTWARE

The cameras are provided with an appropriate software for the ReconFlex™ functionality. The images below show microscopy at the nanoFLeye™.

 

    The left window is used to change the camera settings

    The right window is for adjusting the picture values

     Image 1 is a widefield picture of a µm-calibration slide (10µm steps)

     Image 2 shows a "pseudo" super-resolution accumulation of 15,000 pictures with the corresponding image parameters

 

CONTACT

Surface Concept GmbH

 

Am Sägewerk 23a

55124 Mainz

Germany

 

phone: +49 6131 62716 0

fax:        +49 6131 62716 29

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Formular empfangen.

General specifications of the ReconFlex™ 1900-Series:

 

  sensor technology: CMOS (global shutter)

  optical format: 1/1.2”

  pixel size: 5.86µm

  resolution: 1920 x 1200 (2.3Mpixel)

  quantum efficiency: >75% (@ 525nm)

  read noise: <6e-

  saturation capacity: 32500e-

  dynamic range: 75dB

  frame rate (max. @ full frame): 164fps (@ADC 10bits)

  digital dynamic range sampling up to 32bit

  signal-to-noise ratio: 45dB

  exposure time (min.): 20µs

  trigger in/out: LVTTL (<6µs precision)

 

  data interface: USB3.0

  USB3.0 driver for Windows(R)10, 8, 7 and Linux 64bit operation systems

compatible to the Surface Concept universal software interface

ReconFlex™ 1900

Camera system with integrated blob localization (normal resolution) and high precision trigger system (<6µs)

Applications

  • fast and time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • time correlated microscopy with resolutions of 6µs or above
  • very fast „on Board“ localization microscopy with non-interpolated resolution (at sensor pixel resolution)
  • machine vision

Time-to-Digital

Converter

Super-Resolution

Microscopy Mode

ReconFlex™ 1900T

Camera system with integrated blob localization (normal resolution), high precision trigger system (<6µs) and integrated time to digital converter (TDC, 82ps)

Applications

  • ultra fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • ultra fast time correlated microscopy with resolutions of 82ps or above (signals to the TDC can drive the image sensor shutter)
  • ultra fast time correlated „on Board“ localization microscopy with non-interpolated resolution (at sensor pixel resolution)
  • machine vision

Time-to-Digital

Converter

Super-Resolution

Microscopy Mode

ReconFlex™ 1900S

Camera system with integrated latency free super-resolution microscopy mode based on the ReconFlex™ 1900

Applications

  • real time SPDM, STORM, PALM super-resolution localization microscopy (up to 16 times below the sensor pixel resolution)
  • fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • time correlated microscopy with resolutions of 6µs or above
  • machine vision

Time-to-Digital

Converter

Super-Resolution

Microscopy Mode

ReconFlex™ 1900ST

Camera system with integrated latency free super-resolution microscopy mode with TDC based on the ReconFlex™ 1900T

Applications

  • real time SPDM, STORM, PALM super-resolution localization microscopy (up to 16 times below the sensor pixel resolution)
  • ultra fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
  • all types of optical microscopy
  • ultra fast time correlated microscopy with resolutions of 82ps or above (signals to the TDC can drive the image sensor shutter)
  • machine vision

Production level estimated December 2019

Coming soon (under developement): ReconFlex™ 800-Series

 

  High frame rate cameras (up to 1595Hz @ full frame (800x600pixel))

Integrated normal and super-resolution blob localization and time

 correlation

 

ReconFlex™ 800

ReconFlex™ 800T

ReconFlex™ 800S

ReconFlex™ 800ST

 

These 4 models are corresponding in their special functionality to the 4 models of the ReconFlex™ 1900-Series.

 

Production level estimated from 1st quarter 2020.

The cameras are provided with an appropriate software for the ReconFlex™ functionality. The images below show microscopy at the nanoFLeye™.

 

    The left window is used to change the camera settings

    The right window is for adjusting the picture values

    Image 1 is a widefield picture of a µm-calibration slide (10µm steps)

    Image 2 shows a "pseudo" super-resolution accumulation of 15,000

       pictures with the corresponding image parameters

"On Board" Localization of Local Intensity Peaks (so called "Blobs"), e.g. Coordinate Measurements of Thousands of Local Blobs per Frame without Latency Time

 

   Ideal for Phosphor-Screen or Scintillation Readout

 

   Perfect Requirement for Blinking Super-Resolution

     Microscopy

 

Very Fast and very Accurate Time Stamping of Blob Coordinates

 

"On Board" Accumulation of taken Pictures

 

Image Quality very close to well known sCMOS Camera Types

 

High Frame Rates with very High Dynamics

Production level estimated December 2019

Coming soon (under developement): ReconFlex™ 800-Series

 

  High frame rate cameras (up to 1595Hz @ full frame

     (800x600pixel))

 

  Integrated normal and super-resolution blob

     localization and time correlation

 

ReconFlex™ 800

ReconFlex™ 800T

ReconFlex™ 800S

ReconFlex™ 800ST

 

These 4 models are corresponding in their special functionality to the 4 models of the ReconFlex™ 1900-Series.

 

Production level estimated from 1st quarter 2020.

The cameras are provided with an appropriate software for the ReconFlex™ functionality. The images below show microscopy at the nanoFLeye™.

 

    The left window is used to change the camera

        settings

 

    The right window is for adjusting the picture values

 

    Image 1 is a widefield picture of a µm-calibration

        slide (10µm steps)

 

    Image 2 shows a "pseudo" super-resolution

        accumulation of 15,000 pictures with the

        corresponding image parameters