ReconFlex™
Localization microscopy in general is time and disk space consuming by recording an image stack comprising tens of thousands of images for post-processing of the data to determine the position of each molecule (reconstruction).
ReconFlex™ supports a much faster data analysis for localization microscopy.
Advantages
Enormous Reduction in Data Transfer, Time and Disk Space
Select Fiducial Markers prior to the Measurement to correct Possible Drifts in Real-Time
The User retains Full Control over the Algorithms used in the Reconstruction Mode
"On Board" Localization of Local Intensity Peaks (so called "Blobs"), e.g. Coordinate Measurements of Thousands of Local Blobs per Frame without Latency Time
Very Fast and very Accurate Time Stamping of Blob Coordinates
"On Board" Accumulation of taken Pictures
Image Quality very close to well known sCMOS Camera Types
High Frame Rates with very High Dynamics
Provided with ReconFlex™, nanoFLeye™ opens up a new door to real-time, easy and user-friendly super-resolving localization microscopy.
It offers ultra-high flexibilty in terms of its different modes:
Normal Camera Mode: For Live Imaging, adjusting the Microscope and recording Imaging Stacks
Reconstruction Support Mode: A Fast Detection Mode of Localization Areas by the nanoFLeye™ System
CAMERA MODELS
General specifications of the ReconFlex™ 1900-Series:
sensor technology: CMOS (global shutter)
optical format: 1/1.2”
pixel size: 5.86µm
resolution: 1920 x 1200 (2.3Mpixel)
quantum efficiency: >75% (@ 525nm)
read noise: <6e-
saturation capacity: 32500e-
dynamic range: 75dB
frame rate (max. @ full frame): 164fps (@ADC 10bits)
digital dynamic range sampling up to 32bit
signal-to-noise ratio: 45dB
exposure time (min.): 20µs
trigger in/out: LVTTL (<6µs precision)
data interface: USB3.0
USB3.0 driver for Windows(R)10, 8, 7 and Linux 64bit operation systems
compatible to the Surface Concept universal software interface
Time-to-Digital
Converter
Description
Applications
ReconFlex™ 1900
ReconFlex™ 1900T
ReconFlex™ 1900S
ReconFlex™ 1900ST
Camera system with integrated blob localization (normal resolution) and high precision trigger system (<6µs)
Camera system with integrated blob localization (normal resolution), high precision trigger system (<6µs) and integrated time to digital converter (TDC, 82ps)
Camera system with integrated latency free super-resolution microscopy mode based on the ReconFlex™ 1900
Camera system with integrated latency free super-resolution microscopy mode with TDC based on the ReconFlex™ 1900T
Production level estimated December 2019
Coming soon (under developement): ReconFlex™ 800-Series
High frame rate cameras (up to 1595Hz @ full frame (800x600pixel))
Integrated normal and super-resolution blob localization and time correlation
ReconFlex™ 800
ReconFlex™ 800T
ReconFlex™ 800S
ReconFlex™ 800ST
These 4 models are corresponding in their special functionality to the 4 models of the ReconFlex™ 1900-Series.
Production level estimated from 1st quarter 2020.
Super-Resolution
Microscopy Mode
- fast and time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
- all types of optical microscopy
- time correlated microscopy with resolutions of 6μs or above
- very fast „on Board“ localization microscopy with non-interpolated resolution (at sensor pixel resolution)
- machine vision
- ultra fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
- all types of optical microscopy
- ultra fast time correlated microscopy with resolutions of 82ps or above (signals to the TDC can drive the image sensor shutter)
- ultra fast time correlated „on Board“ localization microscopy with non-interpolated resolution (at sensor pixel resolution)
- machine vision
- real time SPDM, STORM, PALM super-resolution localization microscopy (up to 16 times below the sensor pixel resolution)
- fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
- all types of optical microscopy
- time correlated microscopy with resolutions of 6μs or above
- machine vision
- real time SPDM, STORM, PALM super-resolution localization microscopy (up to 16 times below the sensor pixel resolution)
- ultra fast time correlated phosphor or scintillation readout with „on Board“ recognition of blob coordinates (coordinates x, y, t of all single particle hits are read out directly at the image sensor)
- all types of optical microscopy
- ultra fast time correlated microscopy with resolutions of 82ps or above (signals to the TDC can drive the image sensor shutter)
- machine vision
APPLICATIONS
Localization Microscopy
Localization microscopy in general is time and disk space consuming by recording an image stack comprising tens of thousands of images for post-processing of the data to determine the position of each molecule (reconstruction).
The „Super-resolution Mode“ enables the true localization of single molecules. The locations are determined by the integrated algorithm with a precision down to 20nm.
Blob Finder Mode
The Blob Finder Mode enables the true counting of single light blobs. This evaluates the positions independently on the fluctuations of the MCP amplifi cation. Thus, the Blob Finder Camera is capable of linear rate response down to the lowest count rate range.
Frame triggers are programmable by software or follow external TTL signals. These TTL signals can be measured as a third coordinate with a precision of 82ps with respect to a laboratory reference clock.
Timeresolved Imaging
SOFTWARE
The cameras are provided with an appropriate software for the ReconFlex™ functionality. The images below show microscopy at the nanoFLeye™.
The left window is used to change the camera settings
The right window is for adjusting the picture values
Image 1 is a widefield picture of a µm-calibration slide (10µm steps)
Image 2 shows a "pseudo" super-resolution accumulation of 15,000 pictures with the corresponding image parameters
CONTACT
Surface Concept GmbH
Am Sägewerk 23a
55124 Mainz
Germany
phone: +49 6131 62716 0
fax: +49 6131 62716 29
email: info@surface-concept.de